Extended Data Fig. 5: Physiological and behavioral consequences of targeted manipulation of CaMKII activity the CDNs.
From: Dopamine biases decisions by limiting temporal integration
(a) Constitutively active CaMKII (T287D) has little-to-no effect on the ability of CsChrimson stimulation to evoke calcium transients in the CDNs, as measured by changes in fluorescence of GCaMP6s. Left, middle left: average traces after 2 and 5 seconds of CsChrimson stimulation. Middle right: max fluorescence after stimulation. Right: average residual calcium 15-20 seconds after stimulation. (b) CaMKII T287D in the CDNs cannot suppress the heat threat response with a non-functional catalytic ___domain (K43M). Both the T287D and K43M mutations are contained on the same UAS-CaMKII transgene. (c) CaMKII T287D in the CDNs has no effect on fertility. (d) CaMKII T287D in the CDNs extends mating duration. (e) Overexpression of wildtype CaMKII in the CDNs does not decrease the likelihood of terminating mating in response to a heat threat. (f) Expression of CaMKII T287D selectively in CDN-Gal4 cells that do not also express Tsh-Gal80 prevents the motivational consequences of expression of CaMKII T287D in all CDN-Gal4 cells. (g) Expressing constitutively active CaMKII in the CDNs prevents termination in response to wind. (h) CaMKII knockdown in the CDNs does not alter mating duration. (i) Mating termination is still dependent on CDN electrical activity when CaMKII is knocked down in the CDNs. (j) Left: termination response to 500 ms red light stimulation of the CDNs with CaMKII knockdown. Right: paired pulse response with CaMKII knockdown in the CDNs. (k) Left: termination response to 500 ms red light stimulation of the CDNs with expression of CaMKII T287D. Right: paired pulse response with expression of CaMKII T287D in the CDNs. (l) A single 250 millisecond pulse of CDN stimulation is very unlikely to cause termination regardless of time into mating or CaMKII manipulation. Plotted is the fraction of flies (for cycle lengths: 2.5, 5, and 10 sec) from Fig. 3g that terminated in response to the first of 10 pulses.
