Extended Data Fig. 5: Independent evaluation of type 2 cytokines using flow cytometry and multiplexed secretomic assay.
From: Single-cell CAR T atlas reveals type 2 function in 8-year leukaemia remission
a, Gating strategy employed for flow cytometry data analysis. Live Dead Blue (LDB) was utilized for live cell selection, followed by CD3 + CD14/CD19 − T cell gating and intact, single-cell filtering. CAR (FMC63) expression was employed for the selection of successfully transduced CAR+ cells, with subsequent analysis of CD4+ and CD8+ subpopulations conducted separately. b, Frequency comparison of the type-2-cytokine+ population in CD8 + CAR+ cells between persistence groups in the Discovery Cohort. c, Frequency comparison of the type-2-cytokine+ population in CD4+ or CD8 + CAR+ cells between persistence groups in the Validation Cohort. d, Schematic principle of multiplexed secretomic assay to measure functional cytokines. The diagram was created using BioRender. Scatter plot shows mean ± s.e.m. from n = 42 (b) and n = 40 (c) patients. Significance levels were calculated with two-tailed Mann-Whitney test (b, c).
