Extended Data Fig. 10: Bioprospecting of IsPETase candidates.
From: Global marine microbial diversity and its potential in bioprospecting

a, The distribution of 1,598 IsPETase homologues with Ser-Asp-His catalytic triad across varying marine ecosystems. b and c, Phylogenetic analysis of the PETase candidates (b) and ecosystem origins of different clades (c). Color scheme for the ecosystems in c is the same as that in a. d, Alignment of dsPETases with IsPETase. Arrows indicates β-sheets and helix indicates α-helixes. The Ser-Asp-His catalytic triad is labeled by green circles. The conserved serine hydrolase Gly–x1–Ser–x2–Gly motif is highlighted as purple triangles. The two disulfide bonds found in IsPETase are indicated with brown-colored circles and lines. e, Incubation of GfPET films with 50 nM dsPETases at 37 °C for 48 h under various NaCl concentrations. All reactions were performed in technical triplicate. The bars and circles represent the average and individual values, respectively. Error bars represent the s.d. of the replicated experiments.