Extended Data Fig. 7: Puerarin could not exert inhibitory effects on DMV neurons in Gabra1 cKO mice.
From: A brain-to-gut signal controls intestinal fat absorption
a, Construction strategy of Gabra1 vagal conditional knockout mice. b, Representative GABRA1 fluorescent staining and quantification in DMV of cKO and flox control mice (n = 4 per group). c, Representative whole cell patch-clamp recordings from a DMV neuron of control mouse (left), and a DMV neuron from cKO mouse (right). d-e, Average AP frequency (d), and mean membrane potential (e) of recorded DMV neurons from flox (n = 29) and cKO mice (n = 39). f, AP frequency and percentage of DMV neurons from flox (n = 20) and cKO (n = 21) mice showing puerarin-associated inhibition or non-inhibition. g, The body weight of flox and cKO mice before puerarin and vehicle injection (n = 8 per group). h, The body weight of WGA-con and WGA-4i before CNO application (n = 10 per group). Scale bar, 100 μm (b). Data were analyzed using two-tailed Student’s t test (b and h) and two-tailed Mann-Whitney test (d to e), two-sided Chi-square test (f), one-way ANOVA with Holm-Å Ãdák’s multiple comparisons test (f) and two-way ANOVA with Tukey’s multiple comparisons test (g). Data are presented as mean ± S.E.M. * P < 0.05; ** P < 0.01; *** P < 0.001. Detailed statistics see source data.
