Fig. 2: Temporal order of DNA methylation and chromatin conformation reconfiguration during the differentiation of astrocytes.
From: Temporally distinct 3D multi-omic dynamics in the developing human brain
a–c, UMAP dimensionality reduction of cortical RG-derived neuronal and glial cells. The UMAP is labelled by joint pseudotime scores computed using the fusion of DNA methylation and chromatin confirmation information (a), cell types (b) and sample ages (c). d, Distribution of Pearson’s coefficients for CG methylation–RNA and 3CGS–CG methylation correlations. e,f, Normalized gene body CG methylation (e) and 3CGS (f) for cell-type-specific marker genes. g, Absence of non-CG methylation accumulation at cell-type marker genes. h,i, Dimensionality reduction and pseudotime scores computed from CG methylation in neural progenitor RG-1, astrocyte progenitor RG-2 and astrocyte populations. j,k, Dimensionality reduction and pseudotime scores computed from chromatin conformation in RG-1, RG-2 and astrocyte populations. l, Distinct distributions of pseudotime scores computed from CG methylation or chromatin conformation during astrocyte differentiation. m, Direct comparison of pseudotime scores computed from CG methylation or chromatin conformation in individual cells across astrocyte differentiation, labelled by cell types. n,o, Examples of genes showing CG methylation (n) and 3CGS (o) dynamics during the differentiation of astrocytes.
