Extended Data Fig. 7: Yap-Taz is not necessary for LXR-dependent upregulation of Areg.
From: Liver X receptor unlinks intestinal regeneration and tumorigenesis
(a, d) Schematic representation of the experiment. Briefly, SI crypts from Villin-CreERT2 Yapflox/flox Tazflox/flox were cultured in ENR (a-c) or NR (d-f) media for 7 days and then split and re-plated for secondary organoid cultures. Secondary organoids were stimulated with DMSO or GW3965 and treated with tamoxifen (TAM) for the last 24 h of culture. (b, e) qPCR analysis of Yap1 and YAP-target genes in organoids treated or not with tamoxifen. (c, f) qPCR analysis of Yap1, Abca1, Areg and Ereg in organoids treated with DMSO or GW3965 ± tamoxifen. Data are representative of 4–6 independent experiments with 5–8 mice per group (each dot represents one mouse). Significance was assessed by paired two-sided t test. Part of the schematics in panel a and d was drawn using BioRender.com.
