Extended Data Fig. 6: High PGE2 and low IFN-I instruct an immune evasive TME.
From: Cancer cells impair monocyte-mediated T cell stimulation to evade immunity
a, Gating strategy for the identification of intratumoral myeloid populations. b, Flow cytometry myeloid characterization pre and 72 h post-ACT (n = 6 for RTT ± ACT, IRF37 RTT ± ACT, n = 5 for NTT ± ACT, RTT Ptgs2 KO + ACT, n = 4 RTT Ptgs2 KO). c, Flow cytometry characterization of dendritic cell populations pre and 72 h post-ACT, (n = 5 for NTT, RTT Ptgs2 KO ± ACT, IRF37 RTT + ACT, n = 6 for NTT + ACT, RTT ± ACT, IRF37 RTT). d, Relative frequency of cell types across conditions in YUMM1.7OVA (scRNA-seq), for RTT CTRL the RTT mCherry sample was used. e, Heatmap of scaled gene expression (scRNA-seq) between NTT, RTT CTRL, RTT Ptgs1/2 KO and RTT IRF3/7 for individual cell clusters. f, Flow cytometry characterization of SIINFEKL peptide on MHCI of dendritic cells isolated from tumors and pulsed ex vivo with SIINFEKL peptide (n = 6 tumors/group) and MFI quantification. g, Scheme outlining in vivo NK cell depletion in Rag2−/− mice harboring YUMM1.7OVA RTT Ptgs2 KO tumors. h, Representative plot depicting NK1.1+ cells in NK cell-depleted vs CTRL RTT Ptgs2 KO tumors measured by flow cytometry (left) and quantification of cDCs (n = 4 CTRL and n = 3 anti-NK1.1 treated tumors) (right). i, Representative BLI images (left) and quantification of CD8+ OT-1Luc T cell infiltration by BLI (right), (n = 3 mice for NTT, n = 5 mice for all other groups). j, Tumor response to ACT (n = 5 mice/group). Arrow indicates day of ACT. Bar graphs and growth curves depict the mean ± s.e.m. Data in i depicts the mean ± s.e.m. Statistical analysis was performed with a two-tailed unpaired student’s t-test in h. A one-way ANOVA with Tukey’s multiple comparison in f. Two-way ANOVA with Tukey’s multiple comparison in i. ns = not significant.
