Extended Data Fig. 2: In vitro growth curves after PELO knockout.
From: Synthetic lethality of mRNA quality control complexes in cancer
(a) Growth of sgB2M (black) and sgPELO (blue) models reported in Fig. 1g. n = 2 independent biological experiments, shown as mean ± s.e.m. (b) Effect of sgPELO on normal immortalized cell models, HEMa, P = 0.18; HFF-1, P = 0.09; ARPE-19, P = 0.39; MCF10A, P = 0.85. Negative control: B2M sgRNA. Pan essential control: RAN sgRNA. Data shown as mean ± s.e.m, n = 3 biologically independent replicates. (c) Stable NCI-H1299 sgPELO model generation verified by immunoblot and growth rate of NCI-H1299 wildtype as compared to sgPELO. Data shown as mean ± s.e.m, n = 2 biologically independent experiments.
