Extended Data Fig. 10: A reduced number of colonizations with Aap-sc-TTFC still elicits protective responses against tetanus toxin.
From: Discovery and engineering of the antibody response to a prominent skin commensal
a, Experimental design. SPF mice were colonized with either Aap-sc or Aap-sc-TTFC 13 times (bacterial culture at OD600 = 6) on the head or 2, 5, or 6 times (bacterial culture at OD600 = 12) on the whole body. b, Antibody titres against TTFC in the nasal washes and bronchoalveolar lavage (BAL) fluid. c, Colony-forming units (CFUs) for mice shown in b and in Fig. 4i. d, Experimental design for the challenge experiment shown in e. e, Survival curves after receiving 1000 ng/kg of tetanus toxin. Of note, ~170 h post injection, 1/8 mice in the wTTFC group (5 and 6 colonizations) developed mild tetanus symptoms which never reached the humane endpoint (see *). P values were calculated log-ranked tests. All mice shown from two independent experiments.
