Fig. 1: Poor anti-Salmonella activities of enrofloxacin and ceftriaxone.
From: Limited impact of Salmonella stress and persisters on antibiotic clearance
a, Salmonella survival in mouse spleen after an antibiotic dose (grey symbols show re-analysed data from ref. 12; enrofloxacin (ENR): 1 h, n = 10; 2 h, n = 3; 4 h, n = 6; ceftriaxone (CRO), n = 6) or in Mueller–Hinton broth under normal atmosphere at 37 °C (red symbols (this study); enrofloxacin, n = 5; ceftriaxone, n = 3). Each symbol represents an individual mouse or an independent in vitro culture. Lines connect the geometric means. Two-tailed t-test of log-transformed data. b, Salmonella survival in tissue-mimicking chemostat cultures (grey symbols show re-analysed data from ref. 12; enrofloxacin: 1 h and 2 h, n = 12; 4 h, n = 7; ceftriaxone 1 h, 2 h and 4 h, n = 4) or laboratory conditions (red symbols; number of samples as in a). Each symbol represents an individual chemostat reactor. Lines connect the geometric means. Two-tailed t-test of log-transformed data. c, Survival of wild-type (WT) and indicated mutant Salmonella in spleen. Each symbol represents an individual mouse (enrofloxacin: wild type and lexA3 1 h, n = 3; wild type 4 h, n = 3; tisB, n = 4; Δ3T, n = 3; ceftriaxone: wild type and Δ3T, n = 6). Horizontal bars represent geometric means. Enrofloxacin, one-way ANOVA of log-transformed data with comparisons to wild-type data and Holm–Šídák correction for multiple comparisons; ceftriaxone, two-tailed t-test of log-transformed data. d, Survival of wild-type and indicated mutant Salmonella in chemostat cultures. Each symbol represents an individual chemostat reactor (enrofloxacin: wild type and lexA3 1 h, n = 5; wild type 4 h, n = 16; tisB and Δ3T, n = 4; ceftriaxone: wild type, n = 8; Δ3T, n = 7). Horizontal bars represent geometric means (statistical tests as in c).
