Extended Data Fig. 12: Impact of microenvironment and perturbations on immunomodulatory programs.
From: Programs, origins and immunomodulatory functions of myeloid cells in glioma
a) Experimental design and barplot for evaluations of the impact of epigenetic inhibitors on glioma-associated myeloid cell activity and identity programs. 25 GBOs from MGH630 were pooled per condition and sequenced b) Schematic of the four immunomodulatory activity programs in glioma-associated myeloid cells, along with the microenvironmental associations, TF enrichments, and perturbations shown to induce or reverse program expression. Myeloid cell types depicted in each program quadrant are an approximation of the distribution of cell types expressing the respective program. Program distribution across CNS and non-CNS malignancies also noted. c) Bar plots of gene set enrichments from mouse lineage tracing data (Kirschenbaum et al.). Gene sets derived from Top 100 genes in myeloid cells most correlated with recent infiltration (closer to 12 h) or Top 100 genes most correlated with remote infiltration (being in the tumor for up to 48 h). Gene list taken from Kirschenbaum et al. Supplemental Table 2, sheet “Table_S3_Isotype_Control_Time”. P-value generated by gProfiler79 using default settings with a custom gene set matrix consisting of our cNMF programs. Credit: 96-well plate and PBMC tube, Shalek, A. (2005) https://BioRender.com/b15i535.
