Extended Data Fig. 3: Transcriptional response to cysteine deficiency.
From: Unravelling cysteine-deficiency-associated rapid weight loss
a, GO categories that are significantly enriched among genes up- or down-regulated in liver of KO compared to Het mice on No Cys diet. b,c, RFPKM values for Gdf15 (b) and Fgf21 (c) genes from the livers of mice on the different diets (nāā„ā4 for all groups, See also Supplementary Table 2). d, GO categories that are significantly enriched among genes up- or down-regulated in muscle of KO compared to Het mice on No Cys diet. e-g, Muscle bulk RNA-Seq data presented as a heatmap focusing on: upregulated genes involved in oxidative stress, cellular metabolic process, branched chain amino acid metabolism, NAD, and pantetheinase (e), downregulated genes in extracellular organization and animal organ development (f), and genes in the ISR that appear minimally affected (g) in KO compared to Het mice at Day 2 on No Cys diet (See also Supplementary Table 3). h, GO categories that are significantly enriched among genes up- or down-regulated in epididymal fat pad of KO compared to Het mice on No Cys diet. (See also Supplementary Table 4). i, RFPKM values for Ucp1 gene from epididymal fat pad (nā=ā3 for Het, nā=ā4 for KO). All data are on Day 2 based on mice shown in the RNA-seq schematic in Fig. 3a. Red and blue indicates GO categories among up- and down-regulated genes, respectively. Data are meanāĀ±ās.e.m.
