Extended Data Fig. 7: The killswitch increases the viscosity of NPM1 in vitro droplets. | Nature

Extended Data Fig. 7: The killswitch increases the viscosity of NPM1 in vitro droplets.

From: Probing condensate microenvironments with a micropeptide killswitch

Extended Data Fig. 7

a. Coomassie gel of purified recombinant msfGFP-NPM1. The experiment was performed once. b. Representative images of the in vitro mixing of msfGFP-NPM1 with DMSO, TAMRA-R10, TAMRA-R10-KS, or TAMRA-R10-KSF-to-G peptides. This experiment was performed independently twice with similar results. Scale bar: 5 µm. c. Representative images of the in vitro condensate fusion events at different timepoints for msfGFP-NPM1 mixed with DMSO, TAMRA-R1, TAMRA-R10-KS, or TAMRA-R10-KSF-to-G peptides. The experiment was performed independently three times with similar results. Scale bar: 3 µm. d. Plot of the Shape Aspect Ratio vs. Elapsed Time of in vitro condensate fusion events for msfGFP-NPM1 mixed with DMSO (n = 89), TAMRA-R10 (n = 88), TAMRA-R10-KS (n = 88), or TAMRA-R10-KSF-to-G (n = 87) peptides. The curves show the mean value at each time point; the shade around the curves show the 95% CI. e. Plot of the Relaxation time (τ) vs. Length Scale () of in vitro condensate fusion events for msfGFP-NPM1 mixed with DMSO (n = 89), TAMRA-R10 (n = 88), TAMRA-R10-KS (n = 88), or TAMRA-R10-KSF-to-G (n = 87) peptides. The lines are simple linear regression fits for each condition. f. Plot for ratio of viscosity (η) to surface tension (γ) (inverse capillary velocity) of in vitro condensate fusion events for msfGFP-NPM1 mixed with DMSO (n = 89), TAMRA-R10 (n = 88), TAMRA-R10-KS (n = 88), or TAMRA-R10-KSF-to-G (n = 87) peptides. “n” represents fusion events for each condition examined over three independent experiments. Data are mean ± s.d. P-values are from Dunnett’s multiple T3 comparisons test versus DMSO condition after one-way ANOVA.

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