Fig. 3: In situ photo-L crosslinking MS in E. coli.
From: Protein structure prediction with in-cell photo-crosslinking mass spectrometry and deep learning
a, Distance restraints from in-cell photo-L crosslinking MS mapped onto cellular complexes. b, Distance distributions for photo-L crosslinks mapped onto known structures, taking only a single conformer per protein. Bissulfosuccinimidyl suberate (BS3) and disuccinimidyl sulfoxide (DSSO) distograms are obtained by mapping the crosslinks of Lenz et al. 33. The distograms are derived by accounting for homo-multimers (top) or mapping to only within-chain distances (bottom). c, Distance restraint analysis of outer membrane proteins crosslinked with photo-L. The dot represents the median, and the whiskers represent the 1.5× interquartile range.
