Extended Data Fig. 9: Modeling of pancreatic cancer through organoid transplantation and lentiviral initiation.
From: A prime editor mouse to model a broad spectrum of somatic mutations in vivo
a Stacked bar plots indicating incidence of tumors and metastases in animals orthotopically transplanted with UPEC-KrasG12D (n = 9) and UPEC-KrasG12C (n = 9) prime-edited pancreatic organoids. b Bright-field and fluorescent imaging of an omental metastasis and surrounding tissue in an animal transplanted with UPEC-KrasG12D prime-edited pancreatic organoids. c H&E staining of a representative omental metastasis. Scale bar indicates 100 µm. d Experimental workflow for autochthonous pancreatic tumor modeling through retrograde pancreatic ductal infection of lentivirus templating KrasG12D (n = 4) or KrasY96C (n = 3) pegRNAs. e Representative H&E staining of autochthonous pancreatic adenocarcinoma initiated through pancreatic ductal infection of UPEC-KrasG12D. Scale bars indicate 1 mm and 50 µm, respectively.
