Extended Data Fig. 2: Prime editing enables modeling a broader scope of cancer-associated mutations from residues conserved in mice at various homology stringencies.

a The percentage of mutations, categorized by variant type, that fall in a region of homology as a function of flank size. Flank size is defined as the number of amino acids on either side of the mutant codon that must match between the human and mouse orthologs for the mutated codon to be considered orthologous (for example, a flank size of two would mean that a total of five amino acids–two upstream and two downstream of the mutant codon–would need to match in the human and mouse protein to be considered orthologous.). b Capabilities of prime editing (blue) and base editing (red) to model mutations that derive from a wild-type amino acid residue conserved in mice as a function of conserved flank size (that is, the stringency of homology). All calculations assume a base or prime editor that recognizes only NGG PAMs. c Quantification of the ability of base editing (top) and prime editing (bottom) to model orthologous mutations in mice at different flank size values (2, 3, 5, and 10 from left to right). These plots correspond with the data points in panel (b).