Extended Data Fig. 4: Mismatch repair-evasive edits amplify prime editing efficiency across multiple cancer-associated loci.
From: A prime editor mouse to model a broad spectrum of somatic mutations in vivo
a Design of epegRNAs encoding EgfrL860R (right) or KrasG12C (left) with (bottom) or without (top) silent mutations intended to promote MMR evasion. The black line on the sequence schematics indicates the Cas9 nicking site. b Editing efficiency and indel byproduct frequency of epegRNAs encoding KrasG12C with or without silent mutations in tail tip-derived fibroblasts. Note that the data for KrasG12C without silent edits is also featured in Fig. 3. Data and error bars indicate the mean and standard deviation of three independent transductions. c Editing efficiency and indel byproduct frequency of epegRNAs encoding EgfrL860R with or without silent mutations. Data and error bars indicate the mean and standard deviation of three independent transductions. d Editing efficiency and indel byproduct frequency of epegRNAs encoding KrasG12C with or without silent mutations in pancreatic organoids. Note that the data for KrasG12C without silent edits is also featured in Fig. 3. Data and error bars indicate the mean and standard deviation of three independent transductions.
