Extended Data Fig. 7: Editing dynamics of iBE organoids.
From: Generation of precision preclinical cancer models using regulated in vivo base editing
a. Flow cytometry analysis of three independent pancreatic KP mutant organoid lines integrated with GFPGO reporter following dox treatment for 0-8 days (black), transient exposure for 2h or 12h (grey), or transient exposure then re-treatment at 4 days (green). b. Targeted deep sequencing quantification of target C:G to T:A conversion at the ApcQ1405X locus in 2D small intestinal derived iBE cell line following dox addition for 21 days (dark blue), or transient dox treatment for 3 days and withdrawn for 18 days (light blue). c. Targeted deep sequencing quantification of indel conversion of b. Data are presented as mean values ± s.e.m. (*p<0.05, Student’s t-test) (n=3 independently derived line).
