Extended Data Fig. 10: In situ base editing with iBE by synthetic gRNA delivery drives liver tumors.
From: Generation of precision preclinical cancer models using regulated in vivo base editing
a. HTVI delivery of synthetic gRNAs with SB-Myc as in Fig. 4. BF, H&E images, and IF staining for ß-catenin (green) and glutamine synthetase (GS, red) in livers with tumors. Number of transfected mice with palpable tumors is shown below each column. b. Quantification of target C:G to T:A conversion from tumors described in a). Each point corresponds to an isolated bulk tumor. (n=2-7 mice for a given gRNA target). Individual editing data color-coded by animal in Supplementary Fig. 3. All data are presented as mean values ± s.e.m.
