Extended Data Fig. 3: Visualization of editing by various prime editors using the GFP reporter system. | Nature Biotechnology

Extended Data Fig. 3: Visualization of editing by various prime editors using the GFP reporter system.

From: Prime editing using CRISPR-Cas12a and circular RNAs in human cells

Extended Data Fig. 3

Fields of cells labeled GFP (a), untreated sample (b), and only reporter (c) are controls. d–o, Microscope views of reconstitution of GFP by 12 prime editors. Scale bars, 200 µm. One of three independent experiments is shown (n = 3) in a-o. rCas12a, D156R-H759A-LbCas12a; nCas12a, D156R-R1138A-LbCas12a; rnCas12a, D156R-H759A-R1138A-LbCas12a; CircRNA, circular RNA; RTΔR, M-MLV RTΔRNase H.

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