Extended Data Fig. 6: Heatmap showing afatinib resistance scores of 1,817 protein variants.
From: Saturation profiling of drug-resistant genetic variants using prime editing
These variants were generated by prime editing in exons 18-24 of EGFR in PC-9 cells. Boxes outlined in yellow and gray indicate protein variants causing resistant and intermediate phenotypes, respectively. The numbers at the bottom of each heatmap represent the ___location in the EGFR amino acid sequence. At each position, the amino acid in the reference sequence is shown at the top. Thirty protein variants with P-values of two-sided Fisher’s exact test greater than 0.05 or odds ratios lower than 3 were excluded from the analysis and are shown with a white background. Protein variants for which no pegRNAs were designed are shown as gray boxes.
