Extended Data Fig. 9: Zygosity of prime edits.
From: Saturation profiling of drug-resistant genetic variants using prime editing
a, Distribution of reads for each single cell-derived clone containing the indicated SNVs. ‘SNVs’ (shown in sky blue) indicates reads that contain the indicated SNVs without any other mutations. ‘Wild-type’ (green) indicates reads without the intended SNVs or any other mutations. ‘Other’ (yellow) indicates reads that fall into neither of these categories. The number of analyzed single cell-derived clones n = 51 for G930R, 50 for K754Q, and 38 for C797S in EGFR, 12 for K13* and 17 for A48E in RPL15. The stacked bars shown on the left of each graph represent the reads for the populations from which the clones are derived. b, Zygosity of the prime editing-induced SNVs indicated on the x axis. Given that the PC-9 cells that we used contain six and two copies of EGFR and RPL15, respectively, we classified the intended prime edits as partial gene copy editing if the percentage of reads containing SNVs in a clone ranged from 8.3% (=100/6 × 0.5) to 91.7% (100–8.3%) for EGFR and from 20% to 80% for RPL15.
