Supplementary Figure 5: Validation of pairwise interactions detected by Tri-C.
From: Single-allele chromatin interactions identify regulatory hubs in dynamic compartmentalized domains
Comparison of the Capture-C interaction profile (purple) from the viewpoint of the R2 enhancer in the α-globin locus to pairwise interactions derived from Tri-C data. The first Tri-C profile (maroon) shows pairwise interactions derived from all unique Tri-C reads; the tracks below compare pairwise interactions detected in unique Tri-C reads in which two (red), three (orange) or multiple (yellow) interacting fragments were detected. The interaction profiles are very similar, demonstrating that detection of multiple ligation events simultaneously does not skew the data. The subtle differences between the Capture-C and Tri-C profiles are likely related to the use of different restriction enzymes during 3C library preparation (DpnII in Capture-C; NlaIII in Tri-C). Gene annotation (α-globin genes highlighted in red) and erythroid DNase I hypersensitivity (DHS) are shown at the top. Coordinates (mm9): chr11:32,000,000–32,300,000.
