Fig. 3: Using the WashU Virus Genome Browser to study the prevalence of the S-protein p.Asp614Gly alteration.

a, Default browser view of the SARS-CoV-2 genome. The SARS-CoV-2 genome browser includes various genomic datasets in track format (from top to bottom: gene annotations, sequence diversity, mutation alerts, gene expression, predicted immune epitopes, recombination events and RNA modifications). The p.Asp614Gly alteration within the S protein is circled in red within the sequence-diversity track. b, A zoomed-in view of the p.Asp614Gly alteration circled in a. The ruler track displays a color-coded nucleotide sequence. The sequence-diversity and mutation-alert tracks reveal a high degree of variation across SARS-CoV-2 strains at this ___location. Sequence variation within individual strains is displayed below in the form of SNV tracks, which report variations relative to the reference genome in a color-coded format. Notably, mutations at this position appear to be enriched in non-Asian accessions. Beneath these tracks is a dynamic track showing the weekly percentage of strains with the p.Asp614Gly alteration (beginning on 24 December 2019). Finally, the bottom track displays predicted antigenic linear epitopes that were experimentally identified in SARS-CoV by using T-cell, B-cell and major-histocompatibility-complex ligand assays¹⁶. c, Plot showing the percentage of SARS-CoV-2 isolates in each country that contain the p.Asp614Gly alteration (data retrieved from GISAID, accessed on 19 August 2020)⁸. Countries are ordered by continent and then by date of virus introduction. Countries with fewer than ten cases are excluded. Sample sizes are displayed in parentheses beneath each country. d, Plot showing the percentage of SARS-CoV-2 isolates per week (beginning on 24 December 2019) that contain the p.Asp614Gly alteration. Sample sizes for each week are displayed in parentheses.