Extended Data Fig. 10: Evolutionary analysis of ScID1.1 and ScID1.2.

(a) Alignment of the deduced amino acid sequences of ScID1.1 (ScWN6R01G057200), ScID1.2 (ScWN6R01G057300) and their orthologs in rice (RID1, LOC Os10g28330), maize (ID1, Zm00001d032922), barley (HORVU4Hr1G043540), T. urartu (TuG1812G0600001328), Ae. tauschii (AET6Gv20332200), and the three subgenomes of common wheat (TraesCS6A02G126000, TraesCS6B02G154000, and TraesCS6D02G116300). The nuclear localization signal (NLS), the four zinc-finger domains (ZF1 to ZF4) and the ‘TRDFLG’ motif are labeled. The residues are color coded according to their conservancy. (b) Phylogenetic tree of ID1 proteins from representative grasses as inferred using the Maximum Likelihood method and a JTT matrix-based model in MEGA X website. The tree with the highest log likelihood (−2146.66) is shown. The bootstrap values (1000 times) are shown next to the branches. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. All positions containing gaps and missing data were eliminated (complete deletion option). There were a total of 334 positions in the final dataset.