Fig. 5: Validation of ADM using snRNA and immunofluorescence. | Nature Genetics

Fig. 5: Validation of ADM using snRNA and immunofluorescence.

From: Spatially restricted drivers and transitional cell populations cooperate with the microenvironment in untreated and chemo-resistant pancreatic cancer

Fig. 5

a, UMAP plots of acinar and ductal cells from two cases, HT288P1 and HT412P1. Cells are colored by sample. b, UMAP plots of acinar and ductal cells colored by cell types. c, Gene expression signatures derived from scRNA data of acinar and ductal genes across cell types. Each dot indicates expression of a given gene in an annotated cell cluster. The size indicates the percentage of cells expressing that gene and the color is average expression. ADM cells show expression of both acinar and ductal markers in the snRNA data. d, Immunofluorescence staining of tumor and NAT sections. Amylase stains acinar cells (green), cytokeratin-19 stains ductal cells (red), Ki67 stains proliferating cells (white) and Hoechst stains nuclei (blue). For select sections, individual cells expressing both acinar and ductal markers, indicating ADM, are highlighted by the yellow arrowheads. Acinar cells are denoted with a yellow arrow and ductal cells with an outlined yellow angle. e, Proposed models of PDAC development. Development of PDAC along the spectrum from normal to PDAC in humans was initially suggested to be derived predominantly from ductal origin, but, with evidence of ADM cells in humans, an additional model of transition from acinar origin to PDAC is proposed.

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