Extended Data Fig. 3: Live cell tracking of ecDNA through insertion of Tet-O array into the ecDNA of PC3 cells. | Nature Genetics

Extended Data Fig. 3: Live cell tracking of ecDNA through insertion of Tet-O array into the ecDNA of PC3 cells.

From: The evolutionary dynamics of extrachromosomal DNA in human cancers

Extended Data Fig. 3

a, Representative images of PC3 parental and PC3-TetO cell lines showing extensive MYC amplification on both. PC3-TetO shows significant TetO FISH signal on multiple ecDNA bodies as well. b, PCR amplification of 96-mer TetO repeats. c, PCR amplification of 96-mer TetO repeats from DNA isolated from PC3-TetO cells confirming insertion. d, Sanger sequencing of PCR amplification product from PC3-TetO cells. Both left and right junctions were repaired by homologous recombination at the insertion site.

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