Extended Data Fig. 5: Assessment of NKX6-1 in brain tumors and normal tissues. | Nature Genetics

Extended Data Fig. 5: Assessment of NKX6-1 in brain tumors and normal tissues.

From: K27M in canonical and noncanonical H3 variants occurs in distinct oligodendroglial cell lineages in brain midline gliomas

Extended Data Fig. 5

a Immunohistochemistry staining of NKX6-1 protein in normal pancreas tissue as positive control. Arrowhead in left panel indicates region shown at higher magnification in right panel. b-d. Immunohistochemistry staining of NKX6-1 protein in Histone 3 WT, H3.3G34R, and H3.3K27M high-grade glioma patient tumors. e. Antibody staining of NKX6-1 in human tissues from the Human Protein Atlas. Detection levels for each cell type are indicated below, ‘-’ indicates that NKX6-1 was not detected. Image credit: Human Protein Atlas. Images available from http://v21.proteinatlas.org (links provided in Supplementary Table 19). f. Left: In situ hybridization (ISH) in E13.5 mouse brain from the Allen Brain Atlas (© 2008 Allen Institute for Brain Science. Allen Developing Mouse Brain Atlas. Available from: developingmouse.brain-map.org). Right: quantification of ISH expression levels. g. Left: ISH in P56 mouse brain from the Allen Brain Atlas (© 2004 Allen Institute for Brain Science. Allen Mouse Brain Atlas. Available from mouse.brain-map.org). Right: quantification of ISH expression levels. h. Bulk expression levels of NKX6-1 in adult human tissues from GTEx. Sample sizes for brain tissues are indicated.

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