Extended Data Fig. 5: ABI3 and ABI5 were not the directly targets of SD6 and ICE2.
From: Antagonistic control of seed dormancy in rice by two bHLH transcription factors
a, Yeast one-hybrid interactions between SD6 and ICE2 proteins with two elements, ‘E-box variant’ and ‘Triple G-box’. The interaction between p53 proteins and p53 binding elements was used as a positive control, and mutated versions of the E-box and G-box were used as negative controls. b, Schematic representation of the indicated genes: Vertical light blue bar represents the start codon ATG; Vertical red bar represents the stop codon TGA; Vertical orange bars represent G-box motifs; vertical gray bars represent E-box motifs; horizontal thin black lines represent amplicons assayed for SD6 and ICE2 binding. c,d, Relative ChIP-qPCR enrichment of the indicated promoter regions of ABI3 and ABI5 for SD6 (c) and ICE2 (d). Flag-GFP was served as a negative control. Fragment of ACTIN1 gene body region was used as interval negative control. e,f, The expression level of ABI3 and ABI5 in freshly harvested 21-25 DAP seeds of ZH11, sd6 mutants, and ice2 mutants. Each analysis was repeated with three biological replicates. Data are presented as mean values ± s.d.
