Extended Data Fig. 3: SMARCA4 KO Vero E6 cells are resistant to SARS-CoV-2 infection.
From: Pharmacological disruption of mSWI/SNF complex activity restricts SARS-CoV-2 infection
(a) CRISPR/Cas9-mediated SMARCA4 knockout was confirmed by immunoblot in Vero E6 cells. (b) Vero E6 cells were infected with SARS-CoV-2 at an MOI of 0.2. Cell viability relative to an uninfected control was measured 3 dpi with CellTiter Glo. (c) Vero E6 cells were infected with icSARS-CoV-2 mNG at an MOI of 1. Infected cell frequency was measured by mNeonGreen expression at 2 dpi. (d) SMARCA4 knockout Vero E6 cells were infected with VSV pseudovirus (VSVpp): VSVpp-VSV-G and VSVpp-SARS-CoV-2-S. Luciferase relative to a VSVpp-VSV-G control was measured 1 dpi. (e) Cell proliferation of Vero E6 wild type, SMARCA4 KO and KO rescued with empty vector, SMARCA4 wild type or K785R as measured using Cell Titer Glo. Shown are mean ± SEM. n = 3 biological replicates. Data in (a) is one representative one of three independent experiments. Data in (b-d) were analyzed by one-way ANOVA with Tukey’s multiple comparison test. Shown are mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001. n = 3 biological replicates.
