Extended Data Fig. 1: Bivariate flow karyotype of mitotic metaphase chromosomes isolated from the wheat-Th. elongatum 7D/7el2 translocation line carrying the Sr43 resistance gene. | Nature Genetics

Extended Data Fig. 1: Bivariate flow karyotype of mitotic metaphase chromosomes isolated from the wheat-Th. elongatum 7D/7el2 translocation line carrying the Sr43 resistance gene.

From: The wheat stem rust resistance gene Sr43 encodes an unusual protein kinase

Extended Data Fig. 1

DAPI (x-axis) vs. FITC (y-axis) dot plot was obtained after the analysis of DAPI-stained chromosome suspensions labeled by FISHIS with FITC-conjugated probes for GAA and ACG microsatellites. The 7D/7el2 translocation chromosomes were sorted from the sorting window shown as red rectangle at purities of 60–65%. Inset: 7D/7el2 translocation chromosome after FISH with probes for pSc119.2 repeat (green), Afa family repeat (red) and 45S rDNA (yellow) that was used to identify chromosomes in the sorted fraction. Chromosomes were counterstained by DAPI (blue).

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