Fig. 1: Functional screens identify lineage specificities for chromatin factors in hematopoiesis.
From: In vivo screening characterizes chromatin factor functions during normal and malignant hematopoiesis
a, Schema of the experimental approach used, describing the murine progenitor populations used, their isolation and transduction with the CRISPR library, subsequent differentiation, flow sorting of specific differentiation readouts and read-count based analysis of function. CF, chromatin factor; TF, transcription factor. b, Differentiation systems and FACS-based readouts: (1) self-renewal versus differentiation; (2) lineage priming: mega-erythroid versus myeloid; (3) myeloid differentiation: mature myeloid versus non-myeloid; and (4) terminal myeloid maturation versus immature. c, Averaged lineage scores of ‘differentiation versus self-renewal’ (y axis) versus ‘lineage priming’ (x axis) for 554 genes. Significant hits (n = 93 genes with 50% or more significant guide RNAs (gRNAs) in either comparison) are shown with a red cross. NTC sgRNAs are shown with a blue cross. Data for non-Cas9 cells are shown in the background using a yellow-blue density. d, Lineage scores for chromatin factors grouped on the basis of complex membership. The dot color represents the lineage score, the dot size the percentage of significant guides (Supplementary Table 3). HDAC, histone deacetylase; PRMT, protein arginine methyltransferase. e, Exemplar immunophenotypic validations for chromatin factors in the lineage priming (top) and myeloid differentiation (bottom) systems. SSC-A, side scatter area.
