Extended Data Fig. 3: Characterization of the in vivo Perturb-seq system.
From: In vivo screening characterizes chromatin factor functions during normal and malignant hematopoiesis
(a) Comparison of expression profiles from our in vivo single-cell clusters and external cell-type signatures from Izzo et al, 202056. (b) Number of cells per cell type. (c) UMAP projection of the Lineage- and Lineage+ ckit+ fractions. Color scale represents the number of cells in each area. (d) Number of cells with a sgRNAs targeting specific CFs. CF-KOs for which less than 50 cells (in red) were detected were removed from subsequent analysis. (e) Visualization of TF- and CF-KO patterns derived from in vivo Perturb-seq of Chromatin Regulatory Complexes during lineage specification. The distribution of NTCs is shown as background in grey in all plots. Cells are aggregated and the color of each area represents the density of cells in each area.
