Extended Data Fig. 8: The spatial heterogeneity of LPLC-domains.
From: A spatiotemporal atlas of cholestatic injury and repair in mice
a. Left: spatial visualization of subcluster of LPLC-___domain across all timepoints based on Fig. 1e. Scale bars, 500 μm. Black line indicated the position of median zonation layer. Right: time point compositions of each ___domain labeled in the left panel. Data were presented as mean ± SEM (Section number: D0, n = 2; D8, n = 4; D17, n = 3; R2, n = 4; R7, n = 4; R21, n = 5. Rep1 and Rep2 represent the biological replicates). b. Pearson’s correlation coefficient pairwise matrix between each ___domain. c. Pseudotime trajectory analysis corresponding to the designated domains in Extended Data Fig. 8a from Stereo-seq data via Monocle2. d. Violin plots showing the differential enrichment of LPLC-domain1 (top) or LPLC-domain2 (bottom) genes in LPLC1 and LPLC2 from scRNA-seq. e. Cell score distribution of the major cell types along hepa-___domain, LPLC-___domain subtypes, and chol-___domain at D17. Foldchange indicates the fold change of average scores between two LPLC-___domain subtypes. Min-max normalization was applied before comparison. Cell scores lower than 0.01 were presented in grey. f. Expression of representative common ligands in ___domain of LPLC subtypes. g. Spatial feature plots showing the expression pattern of indicated genes (Top layer, black spots) in LPLC-domain1 and LPLC-domain2 (Bottom layer, green and yellow). h. Expression of specific ligands of LPLC-___domain subtypes in the cell types identified from scRNA-seq at D17. i. Expression of specific receptors in both LPLC subtypes or LPLC2 from scRNA-seq at D17.
