Extended Data Fig. 2: Additional characterization of liver zonation and spatial ___domain.
From: A spatiotemporal atlas of cholestatic injury and repair in mice
a. Spatial visualization of the nine zonation layers from central vein to portal vein in representative Stereo-seq sections at D8, R2, R7, and R21. Scale bars, 500 μm. b. Spatial visualization of the pericentral (CV), midzonal (MZ), and periportal (PV) area in representative Stereo-seq sections at D17. Scale bars, 500 μm. Black line indicated the position of median zonation layer, which matched with the midzonal area. c. Line chart showing zonation score of 9 zonation layers among each replicate in Stereo-seq sections for each timepoint. d. UMAP of unsupervised 17 clusters in Stereo-seq. e. Hierarchical clustering of expression profiles of 17 spatial clusters (Clusters 5 and 16, chol-___domain; Cluster 2, LPLC ___domain; Cluster 9, portal vein-area; Other clusters, hepa-___domain). f. Distinct markers for each ___domain from Fig. 1e (log2foldchange ≥ 0.25, q < 0.05). g. UMAP of annotated domains in Stereo-seq. h. Spatial visualization of canonical signaling pathways on a representative D17 slide. Scale bar, 500 μm for whole view and 100 μm for magnified view. i. Average intensity of canonical signaling pathways in each ___domain on Stereo-seq sections during injury.
