Extended Data Fig. 1: Characterization of the G1E-ER4:SMC2-AID-mCherry cell line.
From: Genome folding principles uncovered in condensin-depleted mitotic chromosomes
a, Left panel: representative image showing the condensation of mitotic chromosomes and correct nuclear localization of SMC2-mAID-mCherry fusion protein. Scale bar: 5 μm. Right panel: representative image showing the correct separation of chromatids during ana/telophase. Scale bar: 5 μm. Three independent experiments replicates were performed. b, Left panel: flow cytometry plots showing the rapid degradation of SMC2-mAID-mCherry fusion protein upon auxin treatment. Right panel: flow cytometry plot showing the shift of cell cycle distribution upon long-term auxin treatment. Two independent experiments were performed. c, Growth curve of G1E-ER4:SMC2-mAID-mCherry cells with or without auxin treatment. Error bar denotes SEM (n = 3). P values were calculated using a two-sided Student’s t test. d, Representative image showing the morphology of mitotic chromosomes after auxin treatment for indicated durations (indicated by yellow arrows). Cells were stained with anti-pMPM2 antibody to indicate mitotic population. Scale bars are indicated in the image. Three independent experiments were performed. e, Bar graph showing the percentage of mitotic cells with extensive chromatid entanglements after auxin treatment for indicated durations. Error bar denotes SEM (n = 3). P values were calculated using a two-sided Student’s t test.
