Extended Data Fig. 2: Purification of condensin-deficient mitotic cells.
From: Genome folding principles uncovered in condensin-depleted mitotic chromosomes
a, Flow cytometry plot showing the gating strategy to purify mitotic cells at different time points after addition of auxin. Cells were sorted by pMPM2, DAPI and mCherry signals. More than two independent experiments were performed. b, Bar graph showing the high stratum-adjusted correlation coefficient for each chromosome (n = 19) between biological replicates for each condition. c, KR balanced Hi-C contact matrices (chr2 vs. chr3) showing the gradual increase of trans-chromosome interactions over time upon condensin loss. d, Dot plot showing the percentage of tran-chromosome interactions for independent biological replicates at each tested time point. n = 2 for SMC2(+), 0.5 h, 1 h and 4 h auxin treated samples. n = 4 for 8 h auxin treated samples.
