Extended Data Fig. 8: Important role of Cplx1 in ultrasonic perception in the cochlea.
From: Complexin-1 enhances ultrasound neurotransmission in the mammalian auditory pathway
(a) UMAP visualization of cochlear cells in each bat species and mouse. Cell types are indicated by colors. HC, Hair cell. BC, Basal cell. IC, Intermedaite cell. MC, Marginal cell. Fibro, Fibroblast. Imm, Immune cell. Endo, Endothelial cell. Sch, Schwann cell. SGN-I, Type I spiral ganglion cell. SGN- II, Type II spiral ganglion cell. SC, Supporting cell. OSC/DC/IPC/IBC, Outer sulcus cell/Deiters’ cell/Inner pillar cel/Inner border cell. DC/OSC, Deiters’ cell/Outer sulcus cell. HeC, Hensen’s cell. (b) Dot plot showing expression patterns of representative marker genes for each cochlear cell type. Dot size and color represent the percentage of marker gene expression (Perc. Expr.) and average expression level (Aver. Expr.), respectively. (c) UMAP visualization of the expression patterns of two marker genes for SGN-I (NEFL) and SGN- II (TMEM132E), respectively. (d) Immunofluorescence staining of CPLX1 in cochleae of Cplx1 KD and NC mice. Scale bar: 100 μm. Cells infected by AAV would turn into green (eGFP), and cells with successful Cplx1 KD would exhibit green but less red (CPLX1 antibody), indicating the success of perturbation experiments. (e) RT-qPCR validation of KD efficiency of Cplx1 in mouse cochlea. Three (Cplx1 KD) or two (NC) biological replicates, each with four technical repeats, were conducted in each sample. Data are mean values ± SD. Two-sided t-test P values are indicated. (f) Representative example of mABR signals in a NC (left) and a Cplx1 KD (right) mouse.
