Extended Data Fig. 7: Compartmentalization and TAD establishment following YY1 depletion.
From: YY1-controlled regulatory connectivity and transcription are influenced by the cell cycle
a, YY1 ChIP–qPCR of synchronized, sorted prometaphase cells after mitosis-to-G1 depletion of YY1 (n = 3 biological replicates). b, Bar plot of the percent of cells in G1 after 2-hour release from nocodazole arrest after mitosis-to-G1 depletion of YY1, as assessed by DAPI signal from flow cytometry (two-sided Mann–Whitney U test). c, Micro-C contact probability curves for all biological replicates. d, Representative Micro-C contact maps with tracks of compartmentalization, with positive eigenvector 1 values (EV1) corresponding to A compartment and negative values corresponding to B compartment. e, Heatmap of Pearson correlations of subcompartment eigenvector 1 (EV1) values between biological replicates. f, Histogram plot of EV1 values after mitosis-to-G1 depletion. g, Histogram of log2 insulation score values for all boundaries detected in the −auxin_m contact map. h, Representative Micro-C contact map annotated with example TAD calls. Tracks show log2 insulation score values. i, Aggregate ___domain plot for all TADs called in control contact map, centered on upstream and downstream boundaries. j, Box plot showing loop strengths of all mid G1-detected YY1−YY1 loops weakened by mitosis-to-G1 depletion of YY1 (two-sided Mann–Whitney U test). k, Pileup plots corresponding to loops included in j.
