Fig. 3: Antagonizing function of MEN1 and MLL1 in regulating cytokine gene signature and TME infiltration.
a, RT–qPCR performed in A549 cells with and without MEN1 deletion coupled with deletion of MAVS and/or cGAS. Mean ± s.e.m. of two biological replicates is shown (unpaired two-tailed Student’s t-test). *P < 0.05, **P < 0.01. b, A549 xenograft tumor growth rate in immunodeficient mice with and without knockout of MEN1 or in combination with MAVS and/or cGAS knockout. Each data point represents mean ± s.e.m. tumor volumes (n = 10 for each arm). Two-way ANOVA was used for statistical analysis. ***P < 0.001, ****P < 0.0001. c, Dot plot showing enriched KEGG terms of mouse differential genes from MEN1 knockout A549 xenografts. d, Quantification of neutrophil infiltration in A549 xenograft tumors with and without MEN1 deletion. Mean ± s.e.m. of neutrophil percentage from 12 tumors is shown (unpaired two-tailed Student’s t-test). e, IHC staining showing percentage of neutrophil or CD8+ T cells in MEN1-high versus MEN1-low tumor samples from a LUAD microarray (TMA). Mean ± s.e.m. of 20 tumors with the highest and the lowest MEN1 TMA scores were assigned to each group. **P < 0.01, ***P < 0.001. f, Representative IHC staining images for MEN1, myeloperoxidase (neutrophil) and CD8 (CD8+ T cell). Scale bars, 200 μm. g, A549 xenograft tumor growth rate in immunodeficient mice with and without knockout of MEN1 or in combination with anti-Ly6G antibody injection. Each data point represents mean ± s.e.m. tumor volumes (n = 5 for each arm). Two-way ANOVA was used for statistical analysis. OR, odds ratio.
