Fig. 4: Immunocompetence-dependent tumor-promoting and -inhibiting function of MEN1 in colon cancer. | Nature Genetics

Fig. 4: Immunocompetence-dependent tumor-promoting and -inhibiting function of MEN1 in colon cancer.

From: In vivo CRISPR screens identify a dual function of MEN1 in regulating tumor–microenvironment interactions

Fig. 4

a, Cell proliferation rate of HCT116 in 2D cell culture with and without knockout of MEN1. NS, not significant. Each data point represents mean ± s.e.m. cell counts (n = 3 for each arm). Two-way ANOVA was used for statistical analysis. b, HCT116 xenograft tumor growth rate in immunodeficient mice with and without knockout of MEN1. Each data point represents mean ± s.e.m. tumor volumes (n = 5 for each arm). Two-way ANOVA was used for statistical analysis. *P < 0.05, **P < 0.01. c, Schematic view of murine CT26 engraftment experiment design. d, Tumor growth in immunodeficient NSG mice inoculated with control (sgEV) or Men1 knockout (sgMen1-1, sgMen1-2) CT26 cells. Each data point represents mean ± s.e.m. tumor volumes (n = 12 for each arm). **P < 0.01. e, Tumor growth in immunocompetent BALB/c mice inoculated with control (sgEV) or Men1 knockout (sgMen1-1, sgMen1-2) CT26 cells. Each data point represents mean ± s.e.m. tumor volumes (n = 5 for each arm). Two-way ANOVA was used for statistical analysis. ***P < 0.001. f, Heatmap showing expression value (z-score based on DESeq normalized RNA-seq counts) of differential genes from Men1 knockout versus control tumors in immunocompetent mice. Two control samples and four Men1 knockout tumors were subjected to RNA-seq analysis. g, GO analysis of differentially expressed genes in Men1 knockout versus control cells was performed and the top five terms are shown. The x axis represents the number of genes. Wald tests defined in DEseq2 were used to calculate P values. h,i, Quantification (h) and representative immunofluorescence images (i) of control (sgCtrl) or Men1 knockout (KO) CT26 cells for the detection of dsRNA. Red signal, dsRNA (J2 antibody); blue signal, DAPI. Scale bars, 20 μm. Each bar in the left-hand panel represents the mean of quantifications from 30 randomly picked fields (unpaired two-tailed Student’s t-test). **P < 0.01, ***P < 0.001. CTCF, corrected total cell fluorescence.

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