Extended Data Fig. 10: Identification of a special structure in the stroma of human prostate.

a, Heatmap showing Pearson’s correlation coefficient between cell proportions of selected cell types in total single cells in scRNA-seq data. b, Heatmap showing Pearson’s correlation coefficient between cell signature intensity and marker genes of IM and RTM in GTEx prostate dataset. Signature scores of specific cell subpopulations were calculated by the ssGSEA algorithm. c, Sapling strategy of the slices V2_3 and V2_4 of patient 2. d, Spatial transcriptomic slices, V2_3, of patient 2 colored by signature scores calculated by AddModuleScore (top), expression level of CHGB and inferred cell fractions in each spot. H&E staining image of the type 1 fibroblast-enriched region was enlarged to visualize the histological feature of the structure (top-left). e, Spatial transcriptomic slices, V2_4, of patient 2 colored by signature scores calculated by AddModuleScore (top), expression level of CHGB (bottom-left) and inferred cell fractions in each spot (bottom-middle and bottom-right). f, Multicolor IF staining of the special structure in human prostate, related to Fig. 6k. Experiment has been repeated three times. g, UMAP of fibroblasts in scRNA-seq data colored by expression level of CHGB in fibroblast from the central zone (left), transition zone (middle) and peripheral zone (right) of human prostate.