Extended Data Fig. 9: LC-HRMS analysis of culture media of S. cacaoi ΔpolQ2, wt, and ΔpolQ2 + polQ2.
From: Cryptic phosphorylation in nucleoside natural product biosynthesis
Shown are EICs (calculated m/z for [M+H]+ ± 5 ppm) for polyoxins A, B, D, F, G, H, I, and J. No polyoxin production was detected in ΔpolQ2 (a), polyoxin A, B, F, and G were found in the wt strain (b), and polyoxin A and F were detected in the ΔpolQ2 + polQ2 strain (c). The observations were reproducible for 2-3 different clones for each mutant strain. The culture was repeated twice for each clone.
