Extended Data Fig. 9: BC1618 increases Ampkα protein levels in mice after dietary restriction.
From: A Fbxo48 inhibitor prevents pAMPKα degradation and ameliorates insulin resistance
a, b, C57BL/6 mice were intraperitoneally (i.p.) administered with vehicle or BC1618 (20 mg/kg) on day 1 and simultaneously chow was removed for overnight starvation. After a 17 h fast, mice were given vehicle or BC1618 i.p. 30 min later, mice were euthanized, PBS was perfused through heart to remove blood. Heart, liver, and skeletal muscle was collected and fresh frozen for tissue homogenization and immunoblotting. The pAmpkα and Ampkα protein levels were quantitated and plotted in b. Densitometry data were corrected to tubulin and normalized to vehicle. Data represented mean +/- SEM (n=4-5 independent mice per group). P-values are indicated as calculated by two-tailed unpaired t-test (B).
