Extended Data Fig. 1: pAmpkα undergoes ubiquitin proteasomal degradation.
From: A Fbxo48 inhibitor prevents pAMPKα degradation and ameliorates insulin resistance
a, BEAS-2B cells were pretreated in glucose free media with increasing concentrations of metformin for 4h. Whole cell extracts were subjected to TUBE agarose beads pull downs (PD) and immunoblotting (Results are representative of three independent experiments). b, Fed and overnight starved mouse liver extracts were subjected to TUBE agarose beads pull down (PD) and immunoblotting (Results are representative of three independent experiments). c, BEAS-2B cells were transfected with either WT or T172A mutant Ampk. Whole cell extracts were subjected to TUBE agarose beads pull down (PD) and immunoblotting. Arrows indicate polyubiquitylated products in each blot (Results are representative of three independent experiments).
