Extended Data Fig. 2: pAmpkα ubiquitylation and degradation is mediated by an F-box protein.
From: A Fbxo48 inhibitor prevents pAMPKα degradation and ameliorates insulin resistance
a, BEAS-2B cells were incubated in glucose free 2% DMEM media containing DMSO, MG132 (20 µM, MG) or MLN4924 (5 µM) for 1.5 h. Cells were then collected for immunoblotting (Results are representative of two independent experiments). b, BEAS-2B cells were preincubated in glucose free 2% DMEM media containing DMSO, MG132 (20 µM) or MLN4924 (5 µM) for 1 h. CHX (40 µg/ml) was then added at indicated time points. Cells were collected at same time for immunoblotting (Results are representative of two independent experiments). c, BEAS-2B cells were incubated in high glucose then switched to glucose free 2% DMEM media for 16 h. Total RNA were purified using Qiagen RNeasy Plus Mini Kit. RNA sequencing was then performed by the Quick Biology commercial service. Differentially expressed genes of F-box protein families were plotted and 30% expression level difference was indicated in the red line cut off (Data are the mean of two biological replicates).
