Extended Data Fig. 10: Schematic representation of RyR modulation by CHL.
From: Structural basis for diamide modulation of ryanodine receptor
Schematic representation of RyR modulation by CHL. Structures under three conditions are compared: 1. activating Ca2+; 2. activating Ca2+ plus ATP and caffeine; 3. activating Ca2+ plus caffeine, ATP, CaM1234 and CHL. The binding of Ca2+ primes the channel, but it mainly stays in the closed states. The binding of ATP and caffeine increases the Po and shift the equilibrium towards the open state. The binding of CHL further increase the Po and stabilizes the open state. The increase in Po correlates with the widening of the channel pore.
