Extended Data Fig. 1: Optimization of the sgRNA for AsCas12f1.
From: Programmed genome editing by a miniature CRISPR-Cas12f nuclease
a, The predicted secondary structure of sgRNA_v1 and the truncation information. The gray shaded tri-guanosine is added to the beginning of sgRNA to maximize the RNA production. b, in vitro dsDNA cleavage assay for different truncations of sgRNA. c, Bacterial genome targeting using different truncations of sgRNA. d, The predicted secondary structure of sgRNA_v2.
