Extended Data Fig. 8: Constitutive activity determinants of C3aR.
From: Revealing the signaling of complement receptors C3aR and C5aR1 by anaphylatoxins
a, Histogram of constitutive activities of C3aR and C5aR1, pcDNA3.0 vector was used as control. Cells were treated with decreasing dose of fosklin. It can be seen that C3aR has high basal activity whereas C5aR1 has no basal activities and behaves like the control. Data shown are means ± S.E.M. from N = two independent experiments performed in technical duplicate. b, Structural superposition of the C3a–C3aR with the apo-C3aR in orthogonal view (left), extracellular view (middle) and intracellular view (right). Helixes are shown as rods. c, Structural determinants affecting the constitutive activity of C3aR. In apo-C3aR, residue R3405.42 forms direct hydrogen bond with Y3936.51. d, Characterization of the mutational effects of critical residues on the basal activation of C3aR, pcDNA3.0 vector was used as control. Cells were treated with 1 μM fosklin. Data shown are means ± S.E.M. from five independent experiments (n = 5) performed in technical duplicate. Data were analyzed by two-side, one-way ANOVA by Dunnett multiple test compared with WT. *P < 0.05; **P < 0.01 and ***P < 0.001 were considered as statistically significant. P = 0.005, P = 0.08, P < 0.001, P < 0.001 from left to right.
