Extended Data Fig. 7: Functional and structural characterization of the lactate-TRIM28 interaction.
From: Chemoproteomic mapping of the glycolytic targetome in cancer cells
(a) Gene expression profile of TRIM28 across given cancer types and paired normal tissues retrieved from the TCGA and the GTEx projects were plotted using GEPIA. (b) GEPIA-based analysis implying that high TRIM28 gene expression level is unfavored for patients’ survival for the examined cancer types using Log-rank test (median cutoff). (c) Summarized Rtreated/control values of the TRPs assigned for lactate via the LFQ-TRAP analysis of human recombinant TRIM28 (upper panel, all peptides shown here are TRPType A candidates) and the multiplexed-TRAP analysis of HCT116 cell lysates (bottom panel, the letter before K denotes the type of the classified TRPs). Data represent the mean ± SEM (n=3 biologically independent samples) and P values were determined using an unpaired two-tailed Student’s t-test.
